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Article

Mutational analysis of the C-terminus of human interferon-gamma.

Protein engineering - 1 Jul 1990

Wetzel R, Perry L J, Veilleux C, Chang G

Abstract excerpt

We have developed an expression/mutagenesis system and a series of screening procedures for the study of structure-function relationships in human interferon-gamma (HuIFN-gamma). Here we report a preliminary evaluation of the C-terminal portion of the molecule. An expression vector, p652trp gamma, was constructed which includes (i) the HuIFN-gamma gene under control of the trp promoter, (ii) elements controlling...

Topics

Amino Acid Sequence
Cloning, Molecular
Codon
DNA
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Genomic Library
Humans
Interferon-gamma
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Sensitivity and Specificity

Structure-Activity Relationship

Source

Identifiers

PMID: 2120696
DOI: 10.1093/protein/3.7.611

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2120696-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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