Article
Single-strand conformation polymorphism analysis of candidate genes for reliable identification of alleles by capillary array electrophoresis.
Electrophoresis - 1 Jan 2005
Kuhn David N, Borrone James, Meerow Alan W, Motamayor Juan C, Brown J Steven, Schnell Raymond J
Abstract excerpt
We investigated the reliability of capillary array electrophoresis-single strand conformation polymorphism (CAE-SSCP) to determine if it can be used to identify novel alleles of candidate genes in a germplasm collection. Both strands of three different size fragments (160, 245 and 437 bp) that differed by one or more nucleotides in sequence were analyzed at four different temperatures (18 degrees C, 25 degrees C,...
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