Article
Single-stranded conformational polymorphism analysis using automated capillary array electrophoresis apparatuses.
BioTechniques - 1 Apr 2003
Baba S, Kukita Y, Higasa K, Tahira T, Hayashi K
Abstract excerpt
We describe a new environment of a single-stranded conformational polymorphism (SSCP) analysis using automated capillary array sequencers (e.g., ABI Prism 3100 and 3700). In this environment, electrophoretic conditions, settings for instrument management, and software for data analysis are adjusted for SSCP analysis. Highly reproducible results are obtained with this new system, and fragments with mutations...
Topics
- Alleles
- DNA
- DNA Mutational Analysis
- Electrophoresis, Capillary
- Gene Expression Profiling
- Gene Frequency
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Reproducibility of Results
- Sensitivity and Specificity
