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Article

Use of capillary array electrophoresis single-strand conformational polymorphism analysis to estimate genetic diversity of candidate genes in germplasm collections.

Methods in enzymology - 1 Jan 2005

Kuhn David N, Schnell Raymond J

Abstract excerpt

Capillary array electrophoresis single-strand conformation polymorphism (CAE-SSCP) analysis provides a reliable high-throughput method to genotype plant germplasm collections. Primers designed for highly conserved regions of candidate genes can be used to amplify DNA from plants in the collection. These amplified DNA fragments of identical length are turned into useful markers by assaying sequence differences by...

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PMID: 15865971
DOI: 10.1016/S0076-6879(05)95015-5

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