Article
Diminished S-phase cyclin-dependent kinase function elicits vital Rad53-dependent checkpoint responses in Saccharomyces cerevisiae.
Molecular and cellular biology - 1 Dec 2004
Gibson Daniel G, Aparicio Jennifer G, Hu Fangfang, Aparicio Oscar M
Abstract excerpt
Cyclin-dependent kinase (CDK) is required for the initiation of chromosomal DNA replication in eukaryotes. In Saccharomyces cerevisiae, the Clb5 and Clb6 cyclins activate Cdk1 and drive replication origin firing. Deletion of CLB5 reduces initiation of DNA synthesis from late-firing origins. We have examined whether checkpoints are activated by loss of Clb5 function and whether checkpoints are responsible for the...
Topics
- CDC2 Protein Kinase
- Cell Cycle Proteins
- Cell Survival
- Checkpoint Kinase 2
- Cyclin B
- DNA
- DNA Damage
- Enzyme Activation
- Gene Deletion
- Genotype
- Green Fluorescent Proteins
- Humans
- Mutation
- Plasmids