Article
The in vivo Pig-a gene mutation assay is useful for evaluating the genotoxicity of ionizing radiation in mice.
Environmental and molecular mutagenesis - 1 Oct 2012
Ohtani Shin, Unno Aiko, Ushiyama Akira, Kimoto Takafumi, Miura Daishiro, Kunugita Naoki
Abstract excerpt
The in vivo Pig-a mutation assay has been adapted for measuring mutation in rats, mice, monkeys, and humans. To date, the assay has been used mainly to assess the mutagenicity of chemicals that are known to be powerful point mutagens. The assay has not been used to measure the biological effects associated with ionizing radiation. In this study, we modified the Pig-a gene mutation assay (Kimoto et al. [2011b]:...
Topics
- Animals
- Cells, Cultured
- Erythrocytes
- Flow Cytometry
- Male
- Mice
- Mice, Inbred C57BL
- Mutagenicity Tests
- Mutation
- Radiation, Ionizing
- Reticulocytes