Article
Identification of multiple SRF N-terminal phosphorylation sites affecting DNA binding properties.
The EMBO journal - 1 Mar 1992
Janknecht R, Hipskind R A, Houthaeve T, Nordheim A, Stunnenberg H G
Abstract excerpt
Human serum response factor (SRF) bearing a histidine tag was expressed using vaccinia virus. The recombinant protein was purified and shown to be phosphorylated mainly in its N-terminal part. The corresponding phosphorylation sites were mapped by microsequencing and also appear to be phosphorylated in endogenous serum response factor. Four phosphorylation sites are located on serines within amino acids 77-85,...
Topics
- Amino Acid Sequence
- Blotting, Western
- DNA
- DNA-Binding Proteins
- Electrophoresis, Gel, Two-Dimensional
- HeLa Cells
- Humans
- Kinetics
- Molecular Sequence Data
- Mutation
- Nuclear Proteins