Article
The phosphorylation status of the serine-rich region of the human cytomegalovirus 86-kilodalton major immediate-early protein IE2/IEP86 affects temporal viral gene expression.
Journal of virology - 1 Feb 2005
Barrasa M Inmaculada, Harel Noam Y, Alwine James C
Abstract excerpt
The 86-kDa major immediate-early protein (IE2/IEP86) of human cytomegalovirus (HCMV) contains a serine-rich region (amino acids 258 to 275) with several consensus casein kinase II (CKII) sites. We performed extensive mutational analysis of this region, changing serines to alternating alanines and glycines. Mutation of the serines between amino acids 266 and 275 eliminated in vitro phosphorylation by CKII. In...
Topics
- Base Sequence
- Cell Line
- Cytomegalovirus
- Gene Expression Regulation, Viral
- Humans
- Immediate-Early Proteins
- Molecular Sequence Data
- Mutation
- Phosphorylation
- Serine
- Trans-Activators