Article
Molecular mechanisms of spontaneous and induced loss of heterozygosity in human cells in vitro.
Somatic cell and molecular genetics - 1 Jan 1992
Li C Y, Yandell D W, Little J B
Abstract excerpt
The human TK6 lymphoblast cell line is heteroallelic at the thymidine kinase (TK) locus, with one functional and one nonfunctional allele. Cells that have undergone loss of heterozygosity (LOH) at TK can be selected and cloned in an in vitro assay. In order to study the extent of LOH, we have ana...
Topics
- Cell Line, Transformed
- Chromosomes, Human, Pair 17
- Clone Cells
- Densitometry
- Ethyl Methanesulfonate
- Heterozygote
- Humans
- Lymphocytes
- Mutation
- Thymidine Kinase