Article
A comparison of induced mutation at homologous alleles of the tk locus in human cells.
Mutation research - 1 Mar 1991
Amundson S A, Liber H L
Abstract excerpt
Using a restriction fragment length polymorphism which can distinguish the two copies of the thymidine kinase (tk) gene in the TK6 human lymphoblastoid cell line, we have identified heterozygous subclones with alternate active alleles. Quantitative mutagenesis studies with X-rays revealed a markedly different response, depending on which homolog carried the active allele. The slopes of the dose-response curves...
Topics
- Alleles
- Blotting, Southern
- Cell Division
- Cell Line
- Dose-Response Relationship, Drug
- Dose-Response Relationship, Radiation
- Ethyl Methanesulfonate
- Heterozygote
- Humans
- Kinetics
- Mutation