Article
Detection of genetic alterations induced by low-dose X rays: analysis of loss of heterozygosity for TK mutation in human lymphoblastoid cells.
Radiation research - 1 May 2002
Morimoto Shigeko, Kato Takeshi, Honma Masamitsu, Hayashi Makoto, Hanaoka Fumio, Yatagai Fumio
Abstract excerpt
To elucidate the genetic influence of low-dose ionizing radiation at the chromosome level, we exposed human lymphoblastoid TK6-20C cells to 10 cGy of X rays. The TK mutation frequency was 5.7 +/- 1.3 x 10(-6) at the background level and 6.9 +/- 2.8 x 10(-6) after X irradiation. Although this small increase was not statistically significant (P = 0.40), we applied multilocus analysis using 4 TK locus markers and 12...
Topics
- Cell Line
- Dose-Response Relationship, Radiation
- Humans
- Loss of Heterozygosity
- Lymphocytes
- Models, Genetic
- Mutation
- Sequence Deletion
- Thymidine Kinase
- Translocation, Genetic
- X-Rays