Article
The efflux of a fluorescent probe is catalyzed by an ATP-driven extrusion system in Lactococcus lactis.
Journal of bacteriology - 1 May 1992
Molenaar D, Bolhuis H, Abee T, Poolman B, Konings W N
Abstract excerpt
Many bacteria, both gram positive and gram negative, extrude in an energy-dependent manner the fluorescent pH indicator 2',7'-bis-(2-carboxyethyl)-5[and -6]-carboxyfluorescein (BCECF) (D. Molenaar, T. Abee, and W. N. Konings, Biochim. Biophys. Acta 1115:75-83, 1991). This efflux was studied in detail in Lactococcus lactis, and several indications that a transport system is involved were found. This transport...
Topics
- Adenosine Triphosphate
- Biological Transport, Active
- Dicyclohexylcarbodiimide
- Drug Resistance, Microbial
- Flow Cytometry
- Fluoresceins
- Hot Temperature
- Lactococcus lactis
- Lactose
- Mutation
- Vanadates
