Yeast tRNA ligase mutants are nonviable and accumulate tRNA splicing intermediates.

Article

Yeast tRNA ligase mutants are nonviable and accumulate tRNA splicing intermediates.

The Journal of biological chemistry - 5 Mar 1992

Phizicky E M, Consaul S A, Nehrke K W, Abelson J

Abstract excerpt

We show here that yeast tRNA ligase protein is essential in the cell and participates in joining together tRNA half-molecules resulting from excision of the intron by the splicing endonuclease. A haploid yeast strain carrying a chromosomal deletion of the ligase gene is viable only if ligase prot...

Topics

Chromosome Deletion
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Mutation
Plasmids
RNA Ligase (ATP)
RNA Splicing
RNA, Transfer
Restriction Mapping
Saccharomyces cerevisiae
Temperature

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