Article
The 2'-5' RNA ligase of Escherichia coli. Purification, cloning, and genomic disruption.
The Journal of biological chemistry - 6 Dec 1996
Arn E A, Abelson J N
Abstract excerpt
An RNA ligase previously detected in extracts of Escherichia coli is capable of joining Saccharomyces cerevisiae tRNA splicing intermediates in the absence of ATP to form a 2'-5' phosphodiester linkage (Greer, C., Javor, B., and Abelson, J. (1983) Cell 33, 899-906). This enzyme specifically ligat...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Phenotype
- RNA Ligase (ATP)
- Sequence Analysis, DNA
- Temperature