Analysis of mutant tRNA gene transcripts in vivo in Saccharomyces cerevisiae by abortive primer extension.

doi:10.1016/0003-2697(91)90132-d

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Article

Analysis of mutant tRNA gene transcripts in vivo in Saccharomyces cerevisiae by abortive primer extension.

Analytical biochemistry - 1 Jul 1991

Wilhelm M L, Wilhelm F X, Ebel J P

Abstract excerpt

When the primer extension of a synthetic oligonucleotide hybridized to a complementary region of RNA is made in the presence of only three deoxyribonucleosides triphosphates, elongation of the primer stops as soon as the missing nucleotide is needed. This abortive primer extension assay has been...

Topics

Base Sequence
Gene Expression
Genes, Fungal
Genes, Suppressor
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
RNA, Fungal
RNA, Transfer
Saccharomyces cerevisiae
Templates, Genetic
Transcription, Genetic
Tyrosine

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