Deletion analysis of a multifunctional yeast tRNA ligase polypeptide. Identification of essential and dispensable functional domains.

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Article

Deletion analysis of a multifunctional yeast tRNA ligase polypeptide. Identification of essential and dispensable functional domains.

The Journal of biological chemistry - 25 Apr 1991

Apostol B L, Westaway S K, Abelson J, Greer C L

Abstract excerpt

Splicing of tRNA precursors in extracts of Saccharomyces cerevisiae requires the action of two enzymes: a site specific endonuclease and a tRNA ligase. The tRNA ligase contains three distinct enzymatic activities: a polynucleotide kinase, a cyclic phosphodiesterase, and an RNA ligase. The polypep...

Topics

3',5'-Cyclic-AMP Phosphodiesterases
3',5'-Cyclic-GMP Phosphodiesterases
Amino Acid Sequence
Chromatography, Affinity
Chromosome Deletion
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Gene Expression Regulation, Bacterial
Gene Expression Regulation, Enzymologic
Genes, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Plasmids

RNA Ligase (ATP)

RNA Splicing

Saccharomyces cerevisiae

Sequence Homology, Nucleic Acid

Source

Open in PubMed

Identifiers

PMID: 1850408

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1850408-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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