Article
A single mutation affects both N-acetylglucosaminyltransferase and glucuronosyltransferase activities in a Chinese hamster ovary cell mutant defective in heparan sulfate biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America - 15 Mar 1992
Lidholt K, Weinke J L, Kiser C S, Lugemwa F N, Bame K J, Cheifetz S, Massagué J, Lindahl U, Esko J D
Abstract excerpt
Mutants of Chinese hamster ovary cells have been found that no longer produce heparan sulfate. Characterization of one of the mutants, pgsD-677, showed that it lacks both N-acetylglucosaminyl- and glucuronosyltransferase, enzymes required for the polymerization of heparan sulfate chains. pgsD-677...
Topics
- Animals
- Autoradiography
- CHO Cells
- Cell Fusion
- Chromatography, High Pressure Liquid
- Cricetinae
- Glucosyltransferases
- Glucuronosyltransferase
- Glycosaminoglycans
- Heparitin Sulfate
- Mutation
- N-Acetylglucosaminyltransferases
- Phenotype