Article
Evidence of bar minigene expression and tRNA2Ile sequestration as peptidyl-tRNA2Ile during lambda bacteriophage development.
Journal of bacteriology - 1 Aug 2004
Oviedo de Anda Norma Angélica, Kameyama Luis, Galindo José Manuel, Guarneros Gabriel, Hernandez-Sanchez Javier
Abstract excerpt
Lambda bacteriophage development is impaired in Escherichia coli cells defective for peptidyl (pep)-tRNA hydrolase (Pth). Single-base-pair mutations (bar(-)) that affect translatable two-codon open reading frames named bar minigenes (barI or barII) in the lambda phage genome promote the development of this phage in Pth-defective cells (rap cells). When the barI minigene is cloned and overexpressed from a plasmid,...
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