Article
Transcription of a bacteriophage lambda DNA site blocks growth of Escherichia coli.
Journal of bacteriology - 1 Feb 1990
Guzman P, Rivera Chavira B E, Court D L, Gottesman M E, Guarneros G
Abstract excerpt
The rap mutation in Escherichia coli prevents the growth of bacteriophage lambda. Phage mutations that overcome rap inhibition (bar) have been mapped to loci in the pL operon. We cloned and sequenced three mutations in two of these loci: barIa to the left arm of the lambda attachment site (attP) and barII in the ssb (ea10) gene. The mutations represent single base-pair changes within nearly identical 16-base-pair...
Topics
- Alleles
- Bacteriophage lambda
- Base Sequence
- DNA, Viral
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Restriction Mapping