A short DNA sequence from lambda phage inhibits protein synthesis in Escherichia coli rap.

Abstract excerpt

The Escherichia coli rap mutant inhibits vegetative growth of bacteriophage lambda. Phage mutations termed bar, which overcome the rap defect, have been mapped to three genetic loci in the pL operon. Plasmids with a lambda wild-type bar DNA segment cloned downstream from an active promoter cannot be maintained in rap mutant bacteria. The viability of a rap mutant strain decreases rapidly after induction of...

Topics

• Bacterial Proteins
• Bacteriophage lambda
• Cloning, Molecular
• DNA, Viral
• Electrophoresis, Gel, Two-Dimensional
• Electrophoresis, Polyacrylamide Gel
• Escherichia coli
• Kinetics
• Molecular Weight
• Mutation
• Plasmids