Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Efficient DNA cassette exchange in mouse embryonic stem cells by staggered positive-negative selection.

Genesis (New York, N.Y. : 2000) - 1 Aug 2004

Long Qiaoming, Shelton Kathy D, Lindner Jill, Jones Julie R, Magnuson Mark A

Abstract excerpt

Recombinase-mediated cassette exchange (RMCE), when applied to mouse embryonic stem (ES) cells, promises to increase the ease with which genetic alterations can be introduced into targeted genomic loci in the mouse. However, existing selection strategies for identifying ES cells in which replacement DNA cassettes from a carrier plasmid have been exchanged correctly into a defined locus are suboptimal. Here, we...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Embryo, Mammalian
Share to Gene Components

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Targeting

Share to Glucokinase

Share to Mutagenesis, Insertional

Share to Plasmids

Share to Recombinases

Share to Stem Cells

Source

Identifiers

PMID: 15286998
DOI: 10.1002/gene.20053

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Efficient DNA cassette exchange in mouse embryonic stem cells by staggered positive-negative selection.PMID 15286998Read ↗

RMCE-ASAP: a gene targeting method for ES and somatic cells to accelerate phenotype analyses.Semantic proximity Read ↗

The loss-of-allele assay for ES cell screening and mouse genotyping.Semantic proximity Read ↗

CRISPR/Cas9-Mediated Insertion of loxP Sites in the Mouse Dock7 Gene Provides an Effective Alternative to Use of Targeted Embryonic Stem Cells.Semantic proximity Read ↗

PCR-based strategy for genotyping mice and ES cells harboring loxP sites.Semantic proximity Read ↗

Rapid confirmation of gene targeting in embryonic stem cells using two long-range PCR techniques.Semantic proximity Read ↗

Generation of knockout alleles by RFLP based BAC targeting of polymorphic embryonic stem cells.Semantic proximity Read ↗

Stable and efficient cassette exchange under non-selectable conditions by combined use of two site-specific recombinases.Semantic proximity Read ↗

Efficient generation of long-distance conditional alleles using recombineering and a dual selection strategy in replicate plates.Semantic proximity Read ↗

Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles.Semantic proximity Read ↗

Mutant loxP vectors for selectable marker recycle and conditional knock-outs.Semantic proximity Read ↗

Mouse galactokinase: isolation, characterization, and location on chromosome 11.Semantic proximity Read ↗

Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.