Article
Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells.
Nucleic acids research - 1 Apr 2009
Nyabi Omar, Naessens Michael, Haigh Katharina, Gembarska Agnieszka, Goossens Steven, Maetens Marion, De Clercq Sarah, Drogat Benjamin, Haenebalcke Lieven, Bartunkova Sonia, De Vos Ilse, De Craene Bram, Karimi Mansour, Berx Geert, Nagy Andras, Hilson Pierre, Marine Jean-Christophe, Haigh Jody J
Abstract excerpt
The ability to rapidly and efficiently generate reliable Cre/loxP conditional transgenic mice would greatly complement global high-throughput gene targeting initiatives aimed at identifying gene function in the mouse. We report here the generation of Cre/loxP conditional ROSA26-targeted ES cells within 3-4 weeks by using Gateway cloning to build the target vectors. The cDNA of the gene of interest can be...
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