Article
A non-radioactive method for the detection of a common mutant allele of aldehyde dehydrogenase 2.
Molecular and cellular probes - 1 Aug 1992
Kawaguchi R, Mukaide M, Hikiji K, Matsunaga T
Abstract excerpt
About 50% of Japanese have been estimated to possess at least one ALDH2*2 allele with a substitution of AAA for GAA at codon 487 of the aldehyde dehydrogenase gene. This mutation is tightly associated with the sensitivity of an individual to alcohol. We developed a method of identifying the ALDH2*2 allele by a non-radioactive technique. DNA from individuals was subjected to polymerase chain reaction in which part...
Topics
- Adamantane
- Aldehyde Dehydrogenase
- Alleles
- Base Sequence
- Humans
- Luminescent Measurements
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Polymerase Chain Reaction