Article
Simple and rapid determination of the acetaldehyde dehydrogenase (ALDH2) genotypes by nonradioactive single-strand conformation polymorphism analysis.
Electrophoresis - 1 Oct 1997
Yuasa I, Umetsu K, Nakagawa M, Ikebuchi J, Inoue T, Irizawa Y
Abstract excerpt
The genotyping of mitochondrial acetaldehyde dehydrogenase (ALDH2) is very important in alcohol studies. We describe an ALDH2 genotyping method based on nonradioactive single-strand conformation polymorphism (SSCP) analysis on mini-gels following amplification with a mutated primer set. The three...
Topics
- Aldehyde Oxidoreductases
- Alleles
- Electrophoresis, Polyacrylamide Gel
- Gene Frequency
- Genotype
- Humans
- Isoenzymes
- Mitochondria, Liver
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational