Article
Improved methods for genotype determination at the ALDH2 locus using PCR by introducing the MBOII recognition site or conducting secondary PCR.
Biochemistry and molecular biology international - 1 Nov 1993
Nakamura K, Iwahashi K, Matsuo Y, Suwaki H, Ichikawa Y
Abstract excerpt
Two convenient methods for determining the ALDH2 genotype using PCR were devised. The first method for distinguishing ALDH2(1) and ALDH2(2) alleles was conducted by introducing a single base change into an oligonucleotide primer for PCR, which produced the MboII recognition site for ALDH2(1) but not for ALDH2(2). The second method was conducted with a secondary PCR using allele-specific primers. Both methods have...
Topics
- Alcohol Dehydrogenase
- Alcoholism
- Alleles
- Asian People
- Base Sequence
- DNA Primers
- Deoxyribonucleases, Type II Site-Specific
- Ethanol
- Flushing
- Genotype
- Humans