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Article

Application of capillary gel electrophoresis to the diagnosis of the aldehyde dehydrogenase 2 genotype.

Journal of chromatography. B, Biomedical applications - 11 Oct 1996

Tomita M, Okuyama T, Hidaka K, Ameno S, Ameno K, Ijiri I

Abstract excerpt

This study dealt with the application of capillary gel electrophoresis (CGE) to diagnosis of the aldehyde dehydrogenase 2 (ALDH-2) genotype. Electrophoresis was performed on a low cross-linked polyacrylamide gel ¿3% T [g acrylamide+g Bis (N,N'-methylenebisacrylamide)], 0.5% C (g Bis/% T)¿ in 100 mM Tris-borate buffer (pH 8.3) at -10 kV with on-column UV detection (260 nm). During the PCR reaction, DNA from the...

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Identifiers

PMID: 8930769
DOI: 10.1016/0378-4347(96)00164-8

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