Article
Spontaneous mutant frequency and mutation spectrum for gene A of phiX174 grown in E. coli.
Environmental and molecular mutagenesis - 1 Jan 2004
Raney Jessica L, Delongchamp Robert R, Valentine Carrie R
Abstract excerpt
The use of transgenic targets for measuring mutant frequencies in mammalian tissue requires an estimate of the mutant frequency that results from recovery of the transgene in bacterial recovery systems. In this study, we have determined the spontaneous mutant frequency, estimated the mutation rate, and ascertained the mutation spectrum for gene A of phiX174 grown in E. coli strain CQ2 from 156 small independent...
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