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Article

Systematic mutagenesis of the Escherichia coli genome.

Journal of bacteriology - 1 Aug 2004

Kang Yisheng, Durfee Tim, Glasner Jeremy D, Qiu Yu, Frisch David, Winterberg Kelly M, Blattner Frederick R

Abstract excerpt

A high-throughput method has been developed for the systematic mutagenesis of the Escherichia coli genome. The system is based on in vitro transposition of a modified Tn5 element, the Sce-poson, into linear fragments of each open reading frame. The transposon introduces both positive (kanamycin resistance) and negative (I-SceI recognition site) selectable markers for isolation of mutants and subsequent allele...

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Share to Genome, Bacterial
Share to Genetic Techniques
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA Transposable Elements

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Share to Mutagenesis, Insertional

Share to Recombination, Genetic

Source

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Identifiers

PMID: 15262929
DOI: 10.1128/JB.186.15.4921-4930.2004
PMCID: PMC451658

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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