Efficient dual-negative selection for bacterial genome editing.

Abstract excerpt

BACKGROUND: Gene editing is key for elucidating gene function. Traditional methods, such as consecutive single-crossovers, have been widely used to modify bacterial genomes. However, cumbersome cloning and limited efficiency of negative selection often make this method slower than other methods such as recombineering. RESULTS: Here, we established a time-effective variant of consecutive single-crossovers. This...

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