Article
Efficient Dual-Negative Selection for Bacterial Genome Editing
2020-03-04
Abstract excerpt
We describe a versatile method for chromosomal gene editing based on classical consecutive single-crossovers. The method exploits rapid plasmid construction using Gibson assembly, a convenient E. coli donor strain, and efficient dual-negative selection for improved suicide vector resolution. We used this method to generate in frame deletions, insertions and point mutations in Salmonella enterica with limited ha...
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Identifiers and source
- Literature Corpus work
- 2081247e-1594-53b4-9a9b-30064a129591
- DOI
- 10.1101/2020.03.03.974816