Article
A simple method for genome-wide screening for advantageous insertions of mobile DNAs in Escherichia coli.
Current biology : CB - 14 May 2002
Edwards Richard J, Sockett R Elizabeth, Brookfield John F Y
Abstract excerpt
Laboratory evolution in Escherichia coli has revealed that fitness typically increases in experimental populations. These changes are sometimes associated with changes in insertion sequence positions, some of which may themselves cause advantageous phenotypes. We have a novel and general method for identifying genes in Escherichia coli, whose knockout by mobile DNA insertions is beneficial in experimental...
Topics
- Chloramphenicol Resistance
- Cloning, Molecular
- DNA Transposable Elements
- Escherichia coli
- Genes, Bacterial
- Genetic Markers
- Genome, Bacterial
- Kanamycin Resistance
- Mutagenesis, Insertional
- Phenotype