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Article

Creation of Staphylococcal Mutant Libraries Using Transposon Tn917.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2016

Rice Kelly C

Abstract excerpt

Non-directed mutagenesis of the staphylococcal genome is a global approach that can be used to identify the genetic basis for phenotypes of interest as well as for identifying regulators of gene expression. One such approach that has been widely used in the study of S. aureus and S. epidermidis is transposon Tn917 mutagenesis to generate random libraries of mutants. This chapter describes the use of plasmid pLTV1...

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PMID: 25646610
DOI: 10.1007/7651_2014_188

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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