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Article

Genetic analysis of prokaryotic and eukaryotic DNA-binding proteins in Escherichia coli.

Nucleic acids research - 15 Aug 1998

Whipple F W

Abstract excerpt

This report describes an Escherichia coli genetic system that permits bacterial genetic methods to be applied to the study of essentially any prokaryotic or eukaryotic site-specific DNA binding protein. It consists of two parts. The first part is a set of tools that facilitate construction of customized E.coli strains bearing single copy lacZYA reporters that are repressed by a specific target protein. The second...

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Identifiers

PMID: 9685485
DOI: 10.1093/nar/26.16.3700
PMCID: PMC147751

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