Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization.

Abstract excerpt

We have developed a new system of chromosomal mutagenesis in order to study the functions of uncharacterized open reading frames (ORFs) in wild-type Escherichia coli. Because of the operon structure of this organism, traditional methods such as insertional mutagenesis run the risk of introducing...

Topics

• Alleles
• Bacterial Proteins
• Cloning, Molecular
• Electroporation
• Escherichia coli
• Escherichia coli Proteins
• Gene Deletion
• Genetic Engineering
• Genetic Vectors
• Genome, Bacterial
• Mutagenesis, Insertional
• Open Reading Frames
• Phenotype
• Polymerase Chain Reaction