In vitro selection of restriction endonucleases by in vitro compartmentalization.

doi:10.1093/nar/gnh096

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Article

In vitro selection of restriction endonucleases by in vitro compartmentalization.

Nucleic acids research - 6 Jul 2004

Doi Nobuhide, Kumadaki Shin, Oishi Yuko, Matsumura Nobutaka, Yanagawa Hiroshi

Abstract excerpt

Restriction endonucleases are widely used in laboratory applications from recombinant DNA technology to diagnostics, but engineering of restriction enzymes by structure-guided design and in vivo directed evolution is at an early stage. Here, we report the use of an in vitro compartmentalization system for completely in vitro selection of restriction enzymes. Compartmentalization of a single gene in a rabbit...

Topics

Amino Acid Sequence
Base Sequence
Catalysis
Cloning, Molecular
DNA Restriction Enzymes
Deoxyribonucleases, Type II Site-Specific
Directed Molecular Evolution
Gene Library
Models, Molecular
Molecular Sequence Data
Mutation

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Identifiers

PMID: 15247328
DOI: 10.1093/nar/gnh096
PMCID: PMC484195

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