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Article

High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions.

Chemistry & biology - 1 Dec 2005

Mastrobattista Enrico, Taly Valerie, Chanudet Estelle, Treacy Patrick, Kelly Bernard T, Griffiths Andrew D

Abstract excerpt

We describe a completely in vitro high-throughput screening system for directed evolution of enzymes based on in vitro compartmentalization (IVC). Single genes are transcribed and translated inside the aqueous droplets of a water-in-oil emulsion. Enzyme activity generates a fluorescent product and, after conversion into a water-in-oil-in-water double emulsion, fluorescent droplets are sorted using a...

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PMID: 16356846
DOI: 10.1016/j.chembiol.2005.09.016

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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