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Article

Directed evolution of streptavidin variants using in vitro compartmentalization.

Chemistry & biology - 22 Sept 2008

Levy Matthew, Ellington Andrew D

Abstract excerpt

We have developed and implemented an in vitro compartmentalization (IVC) selection scheme for the identification of streptavidin (SA) variants with altered specificities for the biotin analog desthiobiotin. Wild-type SA and selected variants bind desthiobiotin with similar affinities (approximately 10(-13) M), but the variants have off rates almost 50 times slower and a half-life for dissociation of 24 hr at 25...

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Identifiers

PMID: 18804035
DOI: 10.1016/j.chembiol.2008.07.017
PMCID: PMC2615486

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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