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Article

A differential cloning procedure for rearranged or altered genomic DNA based on in-gel competitive reassociation.

Advances in biophysics - 1 Jan 1995

Kiyama R, Inoue S, Ohki R, Kikuya E, Yokota H, Oishi M

Abstract excerpt

We have developed a substantially improved differential cloning procedure designed for cloning anonymous altered restriction DNA fragments from higher organisms. The improvements include (i) in-gel dissociation and reassociation of biotinylated restriction digests of target DNA fragments, (ii) replacement of agarose gel by a synthetic gel material for electrophoresis, (iii) use of a reassociation enhancing...

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PMID: 7625271
DOI: 10.1016/0065-227x(95)99389-7

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