A differential cloning procedure for rearranged or altered genomic DNA based on in-gel competitive reassociation.

doi:10.1016/0065-227x(95)99389-7

Back to search

Article

A differential cloning procedure for rearranged or altered genomic DNA based on in-gel competitive reassociation.

Advances in biophysics - 1 Jan 1995

Kiyama R, Inoue S, Ohki R, Kikuya E, Yokota H, Oishi M

Abstract excerpt

We have developed a substantially improved differential cloning procedure designed for cloning anonymous altered restriction DNA fragments from higher organisms. The improvements include (i) in-gel dissociation and reassociation of biotinylated restriction digests of target DNA fragments, (ii) re...

Topics

Animals
Base Sequence
Biophysical Phenomena
Biophysics
Cloning, Molecular
DNA
Gene Rearrangement
Genetic Markers
Humans
Mice
Molecular Sequence Data
Mutation
Recombination, Genetic

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.