Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

In vitro evolution of phi29 DNA polymerases through compartmentalized gene expression and rolling-circle replication.

Protein engineering, design & selection : PEDS - 31 Dec 2019

Sakatani Yoshihiro, Mizuuchi Ryo, Ichihashi Norikazu

Abstract excerpt

Phi29 DNA polymerase is widely used for DNA amplification through rolling-circle replication or multiple displacement amplification. Here, we performed completely in vitro artificial evolution of phi29 DNA polymerase by combining the in vitro compartmentalization and the gene expression-coupled rolling-circle replication of a circular DNA encoding the polymerase. We conducted the experiments in six different...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacillus Phages
Share to Directed Molecular Evolution

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to DNA-Directed DNA Polymerase

Share to Gene Expression

Share to Mutation

Share to Nucleic Acid Amplification Techniques

Source

Identifiers

PMID: 32533140
DOI: 10.1093/protein/gzaa011

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

In vitro evolution of phi29 DNA polymerases through compartmentalized gene expression and rolling-circle replication.PMID 32533140Read ↗

Self-replication of circular DNA by a self-encoded DNA polymerase through rolling-circle replication and recombination.Semantic proximity Read ↗

Directed polymerase evolution.Semantic proximity Read ↗

Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication.Semantic proximity Read ↗

Directed evolution combined with synthetic biology strategies expedite semi-rational engineering of genes and genomes.Semantic proximity Read ↗

Directed evolution of polymerase function by compartmentalized self-replication.Semantic proximity Read ↗

In vitro evolution of phi29 DNA polymerase using isothermal compartmentalized self replication technique.Semantic proximity Read ↗

Advances in in vivo continuous evolution technologies for strain development and enzyme engineering.Semantic proximity Read ↗

Combinatorial Evolution of DNA with RECODE.Semantic proximity Read ↗

Engineered DNA polymerases in biotechnology.Semantic proximity Read ↗

Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.Semantic proximity Read ↗

Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution.Semantic proximity Read ↗

Primer extension mutagenesis powered by selective rolling circle amplification.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.