Article
Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization.
RNA (New York, N.Y.) - 1 Oct 2005
Levy Matthew, Griswold Karl E, Ellington Andrew D
Abstract excerpt
We have used a compartmentalized in vitro selection method to directly select for ligase ribozymes that are capable of acting on and turning over separable oligonucleotide substrates. Starting from a degenerate pool, we selected a trans-acting variant of the Bartel class I ligase which statistically may have been the only active variant in the starting pool. The isolation of this sequence from the population...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.