Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Selection of bacteriophage lambda integrases with altered recombination specificity by in vitro compartmentalization.

Nucleic acids research - 1 Mar 2010

Tay Yvonne, Ho Candice, Droge Peter, Ghadessy Farid J

Abstract excerpt

In vitro compartmentalization (IVC) was employed for the first time to select for novel bacteriophage lambda integrase variants displaying significantly enhanced recombination activity on a non-cognate target DNA sequence. These variants displayed up to 9-fold increased recombination activity over the parental enzyme, and one mutant recombined the chosen non-cognate substrate more efficiently than the parental...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Attachment Sites, Microbiological
Share to Bacteriophage lambda

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Directed Molecular Evolution

Share to Integrases

Share to Plasmids

Share to Recombination, Genetic

Share to Sequence Alignment

Share to Substrate Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 19966270
DOI: 10.1093/nar/gkp1089
PMCID: PMC2831311

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Selection of bacteriophage lambda integrases with altered recombination specificity by in vitro compartmentalization.PMID 19966270Read ↗

Direct selection of trans-acting ligase ribozymes by in vitro compartmentalization.Semantic proximity Read ↗

In vitro selection of restriction endonucleases by in vitro compartmentalization.Semantic proximity Read ↗

Cell-free selection of RNA-binding proteins using in vitro compartmentalization.Semantic proximity Read ↗

High-throughput analyses of a reconstituted diversity-generating retroelement identify intrinsic and extrinsic determinants of diversification.Semantic proximity Read ↗

Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization.Semantic proximity Read ↗

Man-made enzymes--from design to in vitro compartmentalisation.Semantic proximity Read ↗

Context dependence between subdomains in the DNA binding interface of the I-CreI homing endonuclease.Semantic proximity Read ↗

Directed evolution of polymerase function by compartmentalized self-replication.Semantic proximity Read ↗

Improving lambda red genome engineering in Escherichia coli via rational removal of endogenous nucleases.Semantic proximity Read ↗

Development of an in vitro compartmentalization screen for high-throughput directed evolution of [FeFe] hydrogenases.Semantic proximity Read ↗

High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions.Semantic proximity Read ↗

Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selection using in vitro compartmentalisation.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.