Article
Cell-free selection of RNA-binding proteins using in vitro compartmentalization.
Nucleic acids research - 1 Nov 2008
Chen Yu, Mandic Jana, Varani Gabriele
Abstract excerpt
RNA-binding proteins (RBPs) perform many essential functions in the post-transcriptional control of gene expression. If we were able to engineer RBPs with new specificity, it would also become possible to develop new tools to control and investigate gene expression pathways. Molecular evolution methods such as phage display have been introduced to achieve this goal, but the large interface between these proteins...
Topics
- Directed Molecular Evolution
- Gene Library
- Genotype
- Models, Molecular
- Mutation
- Phenotype
- Protein Engineering
- Protein Structure, Tertiary
- RNA
- RNA-Binding Proteins
- Recombinant Fusion Proteins