Article
Exploration of essential gene functions via titratable promoter alleles.
Cell - 9 Jul 2004
Mnaimneh Sanie, Davierwala Armaity P, Haynes Jennifer, Moffat Jason, Peng Wen-Tao, Zhang Wen, Yang Xueqi, Pootoolal Jeff, Chua Gordon, Lopez Andres, Trochesset Miles, Morse Darcy, Krogan Nevan J, Hiley Shawna L, Li Zhijian, Morris Quaid, Grigull Jörg, Mitsakakis Nicholas, Roberts Christopher J, Greenblatt Jack F, Boone Charles, Kaiser Chris A, Andrews Brenda J, Hughes Timothy R
Abstract excerpt
Nearly 20% of yeast genes are required for viability, hindering genetic analysis with knockouts. We created promoter-shutoff strains for over two-thirds of all essential yeast genes and subjected them to morphological analysis, size profiling, drug sensitivity screening, and microarray expression profiling. We then used this compendium of data to ask which phenotypic features characterized different functional...
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