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Article

Bacteria displaying interleukin-4 mutants stimulate mammalian cells and reflect the biological activities of variant soluble cytokines.

Chembiochem : a European journal of chemical biology - 7 Jun 2004

Krause Sebastian, Würdemann Dieco, Wentzel Alexander, Christmann Andreas, Fehr Holger, Kolmar Harald, Friedrich Karlheinz

Abstract excerpt

We describe a novel procedure that allows the rapid determination of cytokine activity on cells that express their cognate receptor. The four-helix bundle cytokine interleukin-4 (IL-4) was inducibly expressed as a fusion with the E. coli outer-membrane protein intimin, such that IL-4 was presented on the surfaces of the bacteria. Expression and accessibility of the cytokine on the cell exteriors were monitored by...

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PMID: 15174163
DOI: 10.1002/cbic.200300837

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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