Article
Multiple sites on IL-8 responsible for binding to alpha and beta IL-8 receptors.
Journal of immunology (Baltimore, Md. : 1950) - 1 Dec 1993
Schraufstätter I U, Barritt D S, Ma M, Oades Z G, Cochrane C G
Abstract excerpt
To define the structural features important for IL-8 binding to its two known receptors, mutants of IL-8 and melanoma growth-stimulating activity (MGSA) and chimerae consisting of segments of these two chemokines were constructed and purified from the pGEX 2T Escherichia coli expression vector. IL-8 alpha and beta receptors were expressed stably and individually in 293 kidney epithelial cells and HL60 human...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Calcium
- Chemokine CXCL1
- Chemokines, CXC
- Chemotactic Factors
- Growth Substances
- Humans
- Intercellular Signaling Peptides and Proteins
- Interleukin-8