Article
RNA primary sequence or secondary structure in the translational initiation region controls expression of two variant interferon-beta genes in Escherichia coli.
The Journal of biological chemistry - 15 Oct 1990
Gross G, Mielke C, Hollatz I, Blöcker H, Frank R
Abstract excerpt
Efficient expression in Escherichia coli (E. coli) of the human interferon-beta gene (IFN-beta) gene and of a chemically synthesized IFN-beta gene variant (506 base pairs; synIFN-beta) adapted to the E. coli codon usage, both fused to the E. coli atpE ribosome-binding site, is controlled either b...
Topics
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Genes, Synthetic
- Genetic Variation
- Humans
- Interferon Type I
- Molecular Sequence Data
- Nucleic Acid Conformation
- Peptide Chain Initiation, Translational
- RNA, Messenger
- Sequence Homology, Nucleic Acid