In vitro secondary structure analysis of mRNA from lacZ translation initiation mutants.

Abstract excerpt

mRNA secondary structure can be an important determinant of the efficiency of translation initiation. To study the effect of secondary structure on translation initiation, in vitro secondary structure analysis was performed on 32 lacZ RNA transcripts that differ in their in vivo translation initiation efficiencies because of mutations. We have shown that well-translated RNA has a relatively unstructured...

Topics

• Base Composition
• Base Sequence
• Cloning, Molecular
• Escherichia coli
• Genetic Vectors
• Lac Operon
• Models, Molecular
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation
• Plasmids