Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Cell-free synthesis of recombinant proteins from PCR-amplified genes at a comparable productivity to that of plasmid-based reactions.

Biochemical and biophysical research communications - 23 Dec 2005

Ahn Jin-Ho, Chu Hun-Su, Kim Tae-Wan, Oh In-Seok, Choi Cha-Yong, Hahn Geun-Hee, Park Chang-Gil, Kim Dong-Myung

Abstract excerpt

The functional stability of mRNA is one of the crucial factors affecting the efficiency of cell-free protein synthesis. The importance of the stability of mRNA in the prolonged synthesis of protein molecules becomes even greater when the cell-free protein synthesis is directed by PCR-amplified DNAs, because the linear DNAs are rapidly degraded by the endogenous nucleases and, thus, the continuous generation of...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Extracts
Share to Cell-Free System

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Endoribonucleases

Share to Plasmids

Share to Protein Biosynthesis

Share to Protein Structure, Secondary

Share to Recombinant Proteins

Share to RNA Stability

Source

Identifiers

PMID: 16263088
DOI: 10.1016/j.bbrc.2005.10.094

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Cell-free synthesis of recombinant proteins from PCR-amplified genes at a comparable productivity to that of plasmid-based reactions.PMID 16263088Read ↗

Cell-free protein synthesis system from Escherichia coli cells cultured at decreased temperatures improves productivity by decreasing DNA template degradation.Semantic proximity Read ↗

Cell-free expression systems for eukaryotic protein productionSemantic proximity Read ↗

Cell-free protein synthesis for proteomics.Semantic proximity Read ↗

Solid-phase translation and RNA-protein fusion: a novel approach for folding quality control and direct immobilization of proteins using anchored mRNA.Semantic proximity Read ↗

Effective Synthesis of mRNA during In Vitro Transcription with Fewer Impurities Produced.Semantic proximity Read ↗

Protein engineering accelerated by cell-free technology.Semantic proximity Read ↗

Segmented poly(A) tails significantly reduce recombination of plasmid DNA without affecting mRNA translation efficiency or half-lifeSemantic proximity Read ↗

PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.Semantic proximity Read ↗

Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategiesSemantic proximity Read ↗

Combining an optimized mRNA template with a double purification process allows strong expression of in vitro transcribed mRNASemantic proximity Read ↗

RNA stem-loop enhanced expression of previously non-expressible genes.Semantic proximity Read ↗

A systematic approach for testing expression of human full-length proteins in cell-free expression systemsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.