Article
Analysis of the germinal center reaction and in vivo long-lived plasma cells.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2004
Toellner Kai-Michael, Khan Mahmood, Sze Daniel Man-Yuen
Abstract excerpt
This chapter describes the analysis of long- and short-lived plasma cells on tissue sections. Mice are immunized with 4-hydroxy-3-nitrophenyl acetyl (NP) coupled to a T-cell-dependent carrier. Antigen-specific germinal center cells and extrafollicular plasma cells are identified by immunohistology on tissue sections. Plasma cells labeled with 5-bromo-2'-deoxyuridine (BrdU) pulses given during different phases of...
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