Article
In situ studies of the primary immune response to (4-hydroxy-3-nitrophenyl)acetyl. III. The kinetics of V region mutation and selection in germinal center B cells.
The Journal of experimental medicine - 1 Oct 1993
Jacob J, Przylepa J, Miller C, Kelsoe G
Abstract excerpt
In the murine spleen, germinal centers are the anatomic sites for antigen-driven hypermutation and selection of immunoglobulin (Ig) genes. To detail the kinetics of Ig mutation and selection, 178 VDJ sequences from 16 antigen-induced germinal centers were analyzed. Although germinal centers appea...
Topics
- Animals
- B-Lymphocytes
- Base Sequence
- DNA, Single-Stranded
- Female
- Genes, Immunoglobulin
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Kinetics
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Mutation
- Nitrophenols
- Phenotype
- Phenylacetates
- Polymerase Chain Reaction
- Spleen